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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
29/02/2008 |
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Data da última atualização: |
05/04/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
EGITO, A. S. do; GIRARDET, J. -M.; LAGUNA, L. E.; POIRSON, C.; MOLLÉ, D.; MICLO, L.; HUMBERT, G.; GAILLARD, J. -L. |
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Afiliação: |
ANTONIO SILVIO DO EGITO, CNPC; J.-M. Girardet, Unité de Recherche sur Animal et Fonctionnalités des Produits Animaux (URAFPA); LUIS EDUARDO LAGUNA, CNPC; C. Poirson, Unité de Recherche sur Animal et Fonctionnalités des Produits Animaux (URAFPA); D. Mollé, Institut National de la Recherche Agronomique; L. Miclo, Unité de Recherche sur Animal et Fonctionnalités des Produits Animaux (URAFPA); G. Humbert, Unité de Recherche sur Animal et Fonctionnalités des Produits Animaux (URAFPA); J.-L. Gaillard, Unité de Recherche sur Animal et Fonctionnalités des Produits Animaux (URAFPA). |
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Título: |
Milk-clotting activity of enzyme extracts from sunflower and albizia seeds and specific hydrolysis of bovine k-casein. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
International Dairy Journal, v. 17, n. 7, p. 816-825, Jul. 2007. |
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DOI: |
10.1016/j.idairyj.2006.09.012 |
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Idioma: |
Inglês |
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Conteúdo: |
Milk-clotting activity found in ammonium sulfate-precipitated protein extracts from Albizia lebbeck and Helianthus annuus seeds was studied. Specific clotting activity of albizia seed extract was 15 times higher than that of sunflower seed extract. Zymogram analysis revealed several proteolytic bands in albizia seed extract and one diffuse proteolytic band for sunflower seed extract. Whole bovine casein was incubated with the plant seed extracts or chymosin and some breakdown products were characterized by reversed-phase high-performance liquid chromatography and electrophoresis. Similar to chymosin, the two seed extracts exhibited proteolytic activity toward ?-casein, ?s-casein and ?-casein, with the highest activity observed for the albizia seed extract. Mass spectrometry analysis showed that the sunflower extract hydrolyzed ?-casein at the Phe105?Met106 bond, as does chymosin. The albizia extract also displayed activity on ?-casein, but the Lys116?Thr117 bond was its preferred target. |
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Palavras-Chave: |
Bovine casein; Cheese; Enzima coagulante; Plant rennet; Sunflower. |
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Thesagro: |
Albizia lebbeck; Caseína; Coagulação; Coalho; Extrato Vegetal; Leite. |
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Thesaurus Nal: |
Albizia; Aspartic proteinases; Chymosin; Cynara cardunculus; Kappa-casein; Proteolysis; Sheep. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02194naa a2200433 a 4500
001 1514197
005 2019-04-05
008 2007 bl uuuu u00u1 u #d
024 7 $a10.1016/j.idairyj.2006.09.012$2DOI
100 1 $aEGITO, A. S. do
245 $aMilk-clotting activity of enzyme extracts from sunflower and albizia seeds and specific hydrolysis of bovine k-casein.$h[electronic resource]
260 $c2007
520 $aMilk-clotting activity found in ammonium sulfate-precipitated protein extracts from Albizia lebbeck and Helianthus annuus seeds was studied. Specific clotting activity of albizia seed extract was 15 times higher than that of sunflower seed extract. Zymogram analysis revealed several proteolytic bands in albizia seed extract and one diffuse proteolytic band for sunflower seed extract. Whole bovine casein was incubated with the plant seed extracts or chymosin and some breakdown products were characterized by reversed-phase high-performance liquid chromatography and electrophoresis. Similar to chymosin, the two seed extracts exhibited proteolytic activity toward ?-casein, ?s-casein and ?-casein, with the highest activity observed for the albizia seed extract. Mass spectrometry analysis showed that the sunflower extract hydrolyzed ?-casein at the Phe105?Met106 bond, as does chymosin. The albizia extract also displayed activity on ?-casein, but the Lys116?Thr117 bond was its preferred target.
650 $aAlbizia
650 $aAspartic proteinases
650 $aChymosin
650 $aCynara cardunculus
650 $aKappa-casein
650 $aProteolysis
650 $aSheep
650 $aAlbizia lebbeck
650 $aCaseína
650 $aCoagulação
650 $aCoalho
650 $aExtrato Vegetal
650 $aLeite
653 $aBovine casein
653 $aCheese
653 $aEnzima coagulante
653 $aPlant rennet
653 $aSunflower
700 1 $aGIRARDET, J. -M.
700 1 $aLAGUNA, L. E.
700 1 $aPOIRSON, C.
700 1 $aMOLLÉ, D.
700 1 $aMICLO, L.
700 1 $aHUMBERT, G.
700 1 $aGAILLARD, J. -L.
773 $tInternational Dairy Journal$gv. 17, n. 7, p. 816-825, Jul. 2007.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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| Registros recuperados : 1.804 | |
| 121. |  | BENITES, V. de M.; NOVOTNY, E. H.; TEIXEIRA, W. G.; MADARI, B. E.; PIMENTA, A. S.; TROMPOWSKY, P. M. Use of charcoal and wood carbonization by-products in agriculture: learning with "terra preta de indio". In: INTERNATIONAL AGRICHAR INITIATIVE (IAI) 2007 CONFERENCE, 2007, Terrigal, Australia. [Abstracts]. Terrigal: IAI, 2007. p. 30-31.| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Arroz e Feijão; Embrapa Solos. |
|    |
| 122. | | BENITES, V. de M.; NOVOTNY, E. H.; TEIXEIRA, W. G.; MADARI, B. E.; PIMENTA, A. S.; TROMPOWSKY, P. M. Use of charcoal and wood carbonization by-products in agriculture: learning with "terra preta de indio". In: INTERNATIONAL AGRICHAR INITIATIVE (IAI) 2007 CONFERENCE, 2007, Terrigal, Australia. [Abstracts]. Terrigal: IAI, 2007. p. 30-31.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 123. | | BENTES, D. S.; RUSCHEL, A. R.; SILVA, W. F. M. da; REIS, L. P.; BARROS, H. C. da C.; SOUSA, J. L. de. Estoque da floresta aos 30 anos após colheita de madeira na área de pesquisa do Km 67 na Floresta Nacional do Tapajós-PA. In: REUNIÃO ANUAL DA SBPC, 62., 2010, Natal. Ciências do mar: herança para o futuro: anais/resumos. [S.l.]: SBPC, 2010.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 125. | | BERENGUER, E.; GARDNER, T. A.; FERREIRA, J. N.; ARAGÃO, L. E. O. C.; NALLY, R. M.; THOMSON, J. R.; VIEIRA, I. C. G.; BARLOW, J. Seeing the woods through the saplings: Using wood density to assess the recovery of human-modified Amazonian forests. Journal of Ecology, v. 106, n. 6, p. 2190-2203, Nov. 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Amazônia Oriental. |
|  |
| 126. | | BERENGUER, E.; GARDNER, T.; FERREIRA, J. N.; ARAGÃO, L.; NALLY, R. M.; THOMSON, J.; VIEIRA, I.; BARLOW, J. Seeing the woods through the saplings: using wood density to assess post-disturbance recovery of human-modified tropical forests. In: ANNUAL MEETING OF THE ASSOCIATION FOR TROPICAL BIOLOGY AND CONSERVATION, 53., 2016, Montpellier. Tropical ecology and society: reconciling conservation and sustainable use of biodiversity: program & abstracts. [S.l.]: ATBC, 2016. p. 313.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 130. | | BLAGITZ, M.; BOTOSSO, P. C.; BIANCHINI, E.; MEDRI, M. E. Crescimento radial do tronco e densidade da madeira de espécies arbóreas da Floresta Estacional Semidecidual do Sul do Brasil. In: CONGRESSO NACIONAL DE BOTÂNICA, 64.; ENCONTRO REGIONAL DE BOTÂNICOS MG, BA E ES, 23., 2013, Belo Horizonte. Anais... Belo Horizonte: SBB, 2013. Disponibilizado online.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Florestas. |
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| 132. | | BOLZON DE MUÑIZ, G. I.; CORDEIRO, M. E.; NISGOSKI, S.; TANOBE, V.; MAGALHAES, W. L. E.; SATYANARAYANA, K. G. Treatment of Schizolobium parahyba var. amazonicum wood (Quanwood) laminates by styrene, furfural alcohol and nano silica is due by the end of this week. In: CONGRESO LATINOAMERICANO DE IUFRO, 3., 2013, San José. Bosques, competitividad y territorios sostenibles. San José: CATIE; [S.l.]: IUFRO, 2013. 1 p. IUFROLAT 2013. Disponibilizado online. Resumen.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Florestas. |
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| 136. | | BOLZON DE MUÑIZ, G. I.; MAGALHAES, W. L. E.; CARNEIRO, M. E.; VIANA, L. C. Fundamentos e estado da arte da espectroscopia no infravermelho próximo no setor de base florestal. Ciência Florestal, Santa Maria, RS, v. 22, n. 4, p. 865-875, out./dez. 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Florestas. |
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| Registros recuperados : 1.804 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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